HSCT: Hematopoietic Stem Cell Transplantation W-CGH: Whole-Comparative Genomic Hybridization FISH: Fluorescent in situ Hybridization DIA: Digital Image Analysis
نویسندگان
چکیده
Whole-comparative genomic hybridization (W-CGH) allows one to identify copy number differences in highly repeated DNAs between two genomes. It allowed the identification of nuclear markers that can be used to distinguish cell populations from different individuals in a chimeric situation. We discuss the reliability of W-CGH accomplished with fluorescent in situ hybridization (FISH) and digital image analysis (DIA) to analyze the degree of chimerism in patients a f t e r allogeneic hematopoietic stem cell transplantation (HSCT).
منابع مشابه
Hematopoietic chimerism after allogeneic stem cell transplantation: a comparison of quantitative analysis by automated DNA sizing and fluorescent in situ hybridization
BACKGROUND: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of post...
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تاریخ انتشار 2008